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Figure 1
EGFP expression in embryonic zebrafish expressing EGFP and rat CYP2E1. (A–D) are bright field images of 32 hpf embryos showing EGFP fluorescence in the lower row (E–H). (A,E): wild-type embryo. (B,F): pT2A plasmid-injected embryo (rat CYP2E1 F0). (C,D,G,H): transgenic embryo (F2) that was obtained via incross mating between F1 [+/−] adult fish. (C,G): EGFP-negative embryo (EGFP [−]). (D,H): Strongly fluorescent embryos (EGFP [+]). Bar indicates 200 µm.
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Full text @ Int. J. Mol. Sci.