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Figure 2
Xenoestrogens phenocopy E2 distal nephron segmentation phenotypes. (A) The 28 ss WT (top), 20 µM ethinylestradiol (EE, middle), or 20 µM genistein (GEN, bottom) treated animals stained via WISH for the DE marker (slc12a1) with the somite marker (smyhc) (left), or the DL marker (slc12a3) (right). Scale bar = 100 µm for lower magnification, scale bar = 50 µm for higher magnification. (B,C) DE and DL domain lengths at 28 ss in micrometers. Data presented on graphs are represented as mean ± SD. **** p < 0.0001 (ANOVA).
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