Fig. 8
(A) Example of a zebrafish injected with a rescue construct imaged under differential interference contrast (DIC) illumination and (B) strong expression of the transgenesis marker, mCherry, in the lens with some autofluorescence from the yolk. Examples of DR images of (C) WT, (D) aqp0a−/−, and (E) aqp0a−/−/aqp0b−/− double mutant 4 dpf lenses in equatorial orientation uninjected and injected with WT MIPfun (F. heteroclitus AQP0) rescue construct Tg(HuβB1cry:MIPfun-IRES-mCherry), and water transport dead construct Tg(HuβB1cry:MIPfunN68Q-IRES-mCherry). Representative lenses of at least four experiments are shown.