ZFIN Image: Hsu et al., 2022, Fig. 6

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Figure Caption

Fig. 6

SIX1 loss alters MYOD1 occupancy at muscle differentiation and stem/oncogenic loci

(A) Peak distribution of the MYOD1 TF in SMS-CTR Scramble and SIX1 KD5 and KD6 cells across promoters (±2.5 kb from annotated TSSs), 5ʹ/3ʹ UTR, gene body (which includes intronic and exonic regions), and distal intergenic/enhancer regions.

(B) Motif analysis of overlapping macs2 MYOD1 peak coordinates; the top 2 motifs are shown.

(C) Heatmaps of MYOD1 signal at annotated MYOD1-bound distal intergenic and promoter regions.

(D) Pathway enrichment of distal intergenic and promoter-bound MYOD1 peaks.

(E) H3K27ac, MYOD1, and MYOG tracks over the MYMK and MYLK2 loci in SIX1 KD and Scramble SMS-CTR cells.

(F) C&R quantitative real-time PCR validation of changes in MYOD1 binding at stem/oncogenic and myogenic differentiation genes that occur in SMS-CTR and RD SIX1 KD cells. Statistical differences for each loci were measured using one-way ANOVA followed by post hoc Dunnett’s multiple comparisons test.

Acknowledgments

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