ZFIN Image: Blum et al., 2023, Fig. 5.

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Figure Caption

Fig. 5.

akt1^E18K mosaic expression restricted to early larval stages is sufficient to cause long bone overgrowth. (A) Schematic of the experimental design to analyze the temporal requirement of PI3K/AKT overactivation in long bone overgrowth. (B) Representative in vivo confocal images of the anal fin endoskeleton [chondrocytes are marked by Tg(sox10:DsRed) (magenta)] in hsp:akt^E18K, ubi:GFP (green) mosaic larva. Heat-shock induced akt1^E18K expression prior to cartilage formation (early expression) caused overgrowth in clone-harboring long bones. In contrast, bone size was unaffected if akt1^E18K was expressed post cartilage formation (late expression). Asterisks mark overgrown bones. SL, standard length in mm. Scale bars: 100 μm.

Acknowledgments

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