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Figure 7
Treatment with GANT61, a small molecule that reduces the number of myelinating oligodendrocytes, also reduces the number of circular profiles in DomA treated fish. (A) Tg(mbp:EGFP) × Tg(mbp:EGFP-CAAX) double transgenic fish were exposed to DomA (0.14 ng) or vehicle at 2 dpf. Myelinating oligodendrocytes in the dorsal spinal cord were counted for control and DomA-exposed fish that were exposed to different concentrations of GANT61 (1.5- 4 dpf). Each point represents the number of oligodendrocytes counted within a 269 μM imaging area in a single fish. (B) Number of circular features in dorsal spinal cord of the same control and DomA exposed fish plotted in (A). (C) The number of circular features per oligodendrocyte (OL) in the dorsal spinal cord of the control and DomA-exposed fish that were exposed to different concentrations of GANT61. (D) The number of circular features plotted against the number of dorsal OLs. (E) Images of control fish exposed to 0 μM (E1), 10 μM (E2), 25 μM (E3), and 40 μM GANT61 (E4). (F) Images of DomA fish exposed to 0 μM (F1), 10 μM (F2), 25 μM (F3), and 40 μM GANT61 (F4). Scale bar = 50 μm.* = p < 0.05, ** = p < 0.01, *** p < 0.001 by generalized mixed models with a Poisson or a negative binomial distribution. Teal asterisks label oligodendrocyte cell bodies. Peach arrows point to circular features. Data were aggregated from two trials. See Supplemental Fig. 4 for data from individual trials.