ZFIN Image: Tonelli et al., 2023, Figure 3

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Figure 3

tmem38b^-/- skeletal phenotype. (A) Growth curves representing WT and tmem38b^-/- standard length measured at 5, 7, 14, 21 dpf, 1, 2, 4 and 6 mpf. At 21 dpf and 1 mpf tmem38b^-/- were significantly shorter than WT. WT n ≥ 13, tmem38b^-/- n ≥ 8. (B) At 21 dpf a significant delay in the inflation of the swim bladder was observed in tmem38b^-/- (n = 27) with respect to WT (n = 28). (C) Representative lateral view (left panels) of 14 dpf alizarin red stained WT and tmem38b^-/- fish. The notochord is indicated by the arrowhead. On the right the graph represents the level of the tip of the notochord mineralization, a delayed mineralization was evident in tmem38b^-/- (tmem38b^-/- n = 17) respect to WT (WT n = 28) Scale bar: 500 µm. (D) Vertebral length and vertebral height measurement in WT and tmem38b^-/- zebrafish. Vertebral length (VL) was reduced in tmem38b^-/- compared to WT at 21 dpf and 1 mpf. Vertebral height (VH) was reduced in tmem38b^-/- compared to WT at 21 dpf, 1 and 2 mpf. 21 dpf: WT n= 29; tmem38b^-/- n= 25; 1 mpf: WT n= 24; tmem38b^-/- n= 18; 2 mpf: WT n= 13; tmem38b^-/- n= 14. *,p < 0.05, **p ≤ 0.01.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)