ZFIN Image: Cao et al., 2023, Fig. 2

Image

Figure Caption

Fig. 2

The expression of Opn1mw and NeuN and the apoptosis in 661W cells. a Images of Opn1mw immunofluorescence staining in uninduced 661W cells (control group), H₂O₂-induced 661W cells cocultured with BV-2 cells following LPS stimulation (LPS + H₂O₂ group) and H₂O₂-induced 661W cells cocultured with BV-2 cells following GW2580 treatment and LPS stimulation (GW2580 + LPS + H₂O₂ group). b, c Statistical analysis of b the ratio of Opn1mw/DAPI area and c the mean intensity of Opn1mw-expressing cells (ANOVA; *P < 0.05, ***P < 0.001). d Images of NeuN immunofluorescence staining in 661W cells from the three groups. e, f Statistical analysis of e the ratio of NeuN/DAPI area and f the mean intensity of NeuN-expressing cells (ANOVA; ***P < 0.001). g Flow cytometry of Annexin V-FITC and propidium iodide (PI) staining in 661W cells from the three groups. h Quantitative analysis of the apoptosis rate (ANOVA; **P < 0.01, ***P < 0.001). Scale bars in a and d: 20 μm

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J Nanobiotechnology