ZFIN Image: Mamot et al., 2021, Fig. 5

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Figure Caption

Fig. 5

Fluorescent mRNA transfection into HeLa cells. (A) Electrophoretic resolution of HPLC-purified mRNAs encoding eGFP (N₃-m⁷GRNA_egfp, N₃-m⁷GRNA_egfp-Cy3, Cy5-m⁷GRNA_egfp and Cy5-m⁷GRNA_egfp-Cy3) or Gaussia luciferase (ARCA-RNA_gluc, ARCA-RNA_gluc-Cy3, N₃-m⁷GRNA_gluc, N₃-m⁷GRNA_gluc-mock, N₃-m⁷GRNA_gluc-Cy3, Cy5-m⁷GRNA_gluc and Cy5-m⁷GRNA_gluc-Cy3) used for HeLa transfections. (B) Relative expression of Gaussia luciferase after mRNA transfection as a function of the presence of 3′ Cy3 modification and 5′ cap structure. (C) Flow cytometry readouts after transfection of fluorescent mRNA encoding eGFP. (D) Time-lapse microscopy images of HeLa cells transfected with Cy5-m⁷GRNA_egfp-Cy3 mRNA. Time scale set for 0 h at the beginning of recording of the images (1 h after transfection start).

Acknowledgments

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