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Figure 2
Effect of G-CSFR truncation mutation on primitive hematopoiesis. Wild-type (wt/wt), heterozygous (wt/mdu26) and homozygous (mdu26/mdu26) mutant csf3r embryos were subjected to WISH at 22 hpf with mpo (A–C), spi1b (E–G) and gata1a (I–K), with representative images shown. Individual embryos were assessed for the number of mpo + (D) or spi1b + (H) cells or the area of gata1a (L) staining, with mean and SEM in red and level of statistical significance indicated (***: p < 0.001, ns: not significant; n=20-30).
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