ZFIN Image: Quadri et al., 2021, Fig. 3

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Figure Caption

Fig. 3 Effect of PA5 on melanoma viability. Melanoma spheroids were treated with PA5 from 24 to 72 h. (a) Pictures of spheroids taken at different time points. (b) Total areas occupied by spheroids were evaluated by ImageJ software. (c) Melanoma spheroids were treated with PA5 (250 nM), and dying cells were stained with PI at 24 and 72 h. Quantification of dying cells is presented as PI staining/spheroids area. Bars = 100 μm. Data represent means of at least three spheroids ± SD of triplicate biological experiments. Student’s t-test was used for statistical analyses of PA5-treated samples compared with NT. ∗∗P < 0.01; ∗0.01 < P < 0.05. h, hour; NT, not treated; PA, protein kinase G activator; PI, propidium iodide.

Acknowledgments

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