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Fig. 4 Relief of EZA toxicity toward otic hair cells by anti-oxidants. Zebrafish larvae (3 dpf) were co-treated with 80 ppb EZA and glutathione (GSH), allopurinol (ALO), or D-methionine (MET) as anti-oxidants, or buthionine sulfoximine (BSO), an inhibitor of GSH synthesis, as a pro-oxidant, and then hair cells in otic neuromasts were visualized with YO-PRO-1 (A) and counted (B). The bars represent means ± SD (n = 3). Asterisks or daggers indicate significant differences from the control or EZA (Student’s t-test, *p < 0.05 vs control, †p < 0.05 vs EZA, ††p < 0.01 vs EZA).
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