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Fig. 2

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ZDB-IMAGE-230114-23
Source
Figures for Katraki-Pavlou et al., 2021
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Figure Caption

Fig. 2

Histological evaluation and angiogenesis in Ptprz1−/− and Ptprz1+/+ mouse hearts. A: representative images at ×40 magnification of H&E staining of heart tissue sections from 3 Ptprz1−/− and 3 Ptprz1+/+ male mice at the age of 3 mo. B: representative images of WGA-stained heart tissue sections from Ptprz1−/− and Ptprz1+/+ male mice. Scale bar corresponds to 50 μm. C and D: the number of cardiomyocytes per mm2 and the CSA of 10 cardiomyocytes per slide was measured in at least 4 different slides from each of 3 different mice from each group. Results are expressed as means ± SD of the number of cardiomyocytes per mm2 (C) or the calculated cardiomyocyte CSA expressed in μm2 (D). E: calculated heart to body ratio expressed as means ± SD (n ≥ 5) at 2 different ages is shown. Statistical analysis was performed by Student’s unpaired t test. Both male (M) and female (F) mice were used, and no difference related to sex has been identified. F: paraffin-embedded hearts from Ptprz1−/− and Ptprz1+/+ male mice at the age of 3 wk or 3 mo were stained with rhodamine-conjugated Griffonia simplicifolia for endothelial cells (red). Nuclei were stained with Draq5 (blue). Representative pictures are shown, and scale bars correspond to 50 μm. G: Griffonia simplicifolia lectin-positive endothelial cells per heart area were measured in 2–4 different fields/photo from 3 different animals from each group. Results are expressed as means ± SD of the vascularized cardiac tissue area in arbitrary units (AU). Statistical analysis in all cases was performed by Student’s unpaired t test. CSA, cross-sectional area; H&E, hematoxylin & eosin ; WGA, wheat germ agglutinin.

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. Physiol. Heart Circ. Physiol.