ZFIN Image: Jim et al., 2022, Figure 2

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Figure Caption

Figure 2

The host’s transcriptional response to infection with pneumolysin-positive or pneumolysin-negative S. pneumoniae D39V

(A) Volcano plot from DESeq2 analysis of RNA pools from zebrafish larvae infected with the pneumolysin-deficient S. pneumoniae D39V mutant strain (PLY−) or the complemented S. pneumoniae D39V mutant strain (PLY+) at 8 hpi. The presence of pneumococcal pneumolysin activates a multitude of host genes in response to S. pneumoniae infection (FC > 1.5, q < 0.05).

(B) Gene expression correlation between RNA sequencing (RNA-seq) and RT-qPCR data; each dot represents a single gene; correlation co-efficient (Pearson) and linear regression are indicated.

(C) Gene Ontology enrichment analysis after DESeq2 analysis showed significant enrichment of several immunomodulatory pathways. Note that an individual gene can be part of multiple terms.

(D) Gene set enrichment analysis after DESeq2 analysis showed enrichment of several immunomodulatory pathways in the presence of pneumolysin, whereas the phagolysosome pathway and many metabolic pathways were enriched in the absence of pneumolysin (FDR < 0.50).

Acknowledgments

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