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Figure 2
(A) Maximum intensity projection (50 planes, 50 μm) of chx10:Red with a single vsx1 GFP+ clonal pair. The inter-soma distance between the GFP-only sister neuron to the GFP/Red co-labeled V2a neuron (dark red arrow) is smaller than the distance between non-sister neurons (white arrows). (B) For each clonal pair in either the Chx10 reporter line (n = 27) or the Gata3 reporter line (n = 24), the 3D distance between the two sister neurons (GFP-GFP, red) and the median 3D distance between one sister neuron and its non-sister neurons in the same segment (GFP-Red). Black dot indicates median and lines show 5th–95th percentiles. (C) Paired line plot of inter-soma distances of individual vsx1 GFP+ sister pairs first imaged at 24 hr post fertilization (hpf) and later reimaged at 48 hpf (left) (n = 14) or first imaged at 48 hpf and later reimaged at 96 hpf (right) (n = 66). Red values indicate median distances at each time point. (D) Scatterplot of normalized dorsal (1)-ventral (0) soma position for each of 27 sister V2a/b neurons. Dashed line indicates unity. Typically, the V2b neuron was located more dorsally than the V2a neuron. Black dot indicates the median V2a/b pair position. **Wilcoxon signed-rank test, p=5.2 × 10–4, paired t-test, n = 27 pairs. Source data for this figure are given in Figure 2—source data 1.
Sister V2a/b neurons remain in close proximity to each other.