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Figure 2 Overlays of osteoclasts from imaged individuals shows patterns in distribution. Three individuals were size-matched, stained and live-imaged to create each representation, all scale bars represent 200μm. (A) Diagram representing the methodology used to combine the three individuals in each image. (B) Ventral imaging shows that osteoclasts are distributed along the branchiostegal rays (bsr) throughout all of the tracked developmental timepoints, in addition concentrations of osteoclast can be seen on the mandibles (m) which house lateral line neuromasts (C) Lateral imaging of osteoclasts shows the same distributions as seen on the branchiostegal rays and mandibles but 6.8mm and 8mm images show concentrations of osteoclasts specifically around the hyomandibular foramina (hmf) and on the opercle (op) (D) Dorsal imaging of osteoclasts shows that osteoclasts are not highly active prior to growth of the frontal (f) and parietal bones (pa), with a notable concentration of osteoclasts around the supraorbital lateral line canals which run through the frontal bones around 10mm and 12mm which is comparatively reduced by 14mm, a detailed comparison of individuals at 12mm and 14mm is shown in unprocessed form. Abbreviations: aa (anguloarticular), ar (arches), bsr (branchiostegal ray), ch (ceratohyal), d (dentary), en (entopterygoid), eo (epioccipital), e (ethmoid), eoc (exoccipital), ff (facial nerve foramen), f (frontal), hm (hyomandibula), iop (interopercle), k (kinethmoid), le (lateral ethmoid), m (maxilla), op (opercle), p (parasphenoid), pa (parietal), pe (preethmoid), pm (premaxilla), pop (preopercle), pt (posttemporal), pto (pterotic), sph (sphenotic), se (supraethmoid), soc (supraocciptal), so (supraocciptal), so (supraorbital), sop (subopercle), st (supratemporal).