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Figure 2
Developmental expression of putative zebrafish Il1r1. (A) RT-PCR from WT embryos at 0-3 dpf showed expression of cabz beginning at 1 dpf, maternal expression of zmp at 0 dpf, and actin beta 1 (actb1) transcript as an experimental control. Molecular weight markers are shown on the right. (B) Whole-mount in situ hybridization using antisense DIG-labeled riboprobes against cabz or zmp transcripts showed unrestricted expression of both transcripts at 1, 2, and 3 dpf. Scale bar in (B) is 200 µm.
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