ZFIN Image: Van Dycke et al., 2021, Fig. 3

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Figure Caption

Fig. 3

Compound 4 and rupintrivir block the norovirus protease. (A) The FRET signal was lost upon co-transfection with the wild-type (WT) or a mutated protease (A105V, I109V, A105V/I109V). The signal remained upon co-transfection with the inactive (H30A) protease. (B) Western blot of HEK293T cells co-transfected with the GI, GII or GV WT or mutant proteases along with the FRET construct. Cleavage was observed in the WT and mutant proteases in all genogroups. EV: empty vector. (C) Dose–response curves of compound 4 and rupintrivir on the WT proteases. Mean values ±SEM are presented, Mann–Whitney test **** p < 0.0001, ns: not significant.

Acknowledgments

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