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Fig. 3
LCC-09 inhibits activation of NFκB and STAT1, and ROS generation in endothelial cells. HUVECs were pre-treated with LCC-09 and control DMSO for 30 mins at the indicated concentrations and then stimulated with TNFα (5 ng/mL). (A and B) Cell lysates were subjected to Western blotting. (C) ChIP was performed to examine the binding of NFκB to E-selectin promoter 2 h after TNFα stimulation. (D and E) Cells were stained with Cellrox Green and imaged (D). Fluorescent intensity was measured with a SpectraMax plate reader and compared (E) 30 mins after TNFα stimulation. *p<0.05; **p<0.01; ***p<0.001.
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Full text @ J Inflamm Res