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Fig. 1
LCC-09 reduces TNFα-induced leukocyte adhesion on endothelial cells. (A) Chemical structure of LCC-09. (B–D) HUVECs were pre-treated with LCC-09 or control DMSO vehicle control for 30 mins at the indicated concentrations and then stimulated with TNFα (5 ng/mL) for 6 h. qPCR was performed (N=4 per group) and expressed as relative folds of control group, which was normalized to 1. (E and F) HUVECs were co-cultured with LCC-09 (5 μM) or DMSO vehicle control for 30 mins and then stimulated with TNFα (5 ng/mL) for 20 h. Then HUVECs were co-cultured with CMFDA-labeled monocytic THP1 cells for 30 mins and adhered monocytes were imaged (D) and counted (E). *p<0.05; **p<0.01.