ZFIN Image: Boezio et al., 2022, Fig. 3.

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Figure Caption

Fig. 3.

Epicardial cells are required for ventricular cardiomyocyte growth during early cardiac morphogenesis, but are dispensable at later time points. (A) Schematic of the MTZ treatment protocol. (B-C′,E-F′) Confocal images of 144 hpf Tg(myl7: EGFP-HRAS); TgBAC(tcf21:mCherry-NTR) DMSO and MTZ-treated larvae. Green, 3D reconstruction of epicardial coverage showing a partial recovery of EpiCs in 52-96 hpf MTZ-treated larvae, and a clear reduction in 96-144 hpf MTZ-treated larvae. (D) Quantification of ventricular volume at 144 hpf following epicardial regeneration or late epicardial ablation. (G-I) Confocal images and quantification of 144 hpf Tg(myl7:mCherry-CAAX); TgBAC(tcf21:NLS-EGFP); TgBAC(tcf21:mCherry-NTR) DMSO and MTZ-treated larvae, showing the reduction of tcf21⁺ EpiCs attached to the ventricular myocardium after their late-onset ablation. Controls were pooled, including tcf21:NTR⁺ DMSO-treated and tcf21:NTR⁻ MTZ-treated larvae (see Materials and Methods). Data show the mean±s.d. (D,I) and P-values are from one-way ANOVA with Dunnett's post hoc test (D) or unpaired two-tailed t-test (I). A, atrium; V, ventricle.

Acknowledgments

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