ZFIN Image: Davison et al., 2022, Figure 2

Image

Figure Caption

Figure 2

Simultaneous loss of Slit2 and Slit3 causes retinal axon misprojections at the optic chiasm II: anterograde axon labeling analysis. RGC axons from both eyes were anterogradely labeled through DiI or DiO injection into the retina, and images show the optic chiasm region (ventral view). (A–E) Maximum intensity z-projections of the midline region of 48 hpf of non-injected wild-type (A), slit2−/−^mz (B), slit3 crispant (C) and slit2−/−^mz;slit3 crispant (D,E) embryos. Arrowheads: projections into the ipsilateral optic tract. Arrows: projections into the contralateral optic nerve. (F). Diagrams summarizing the main phenotypic features of each experimental situation shown in this and the previous figures. NR: neural retina; OC: optic chiasm; ON: optic nerve; OT: optic tract. Scale bars: 30 μm. See Video S2.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J Dev Biol