ZFIN Image: Cai et al., 2021, Fig. 6

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Fig. 6

DHX15 R222G is not as effective as DHX15 in restoring HSPC failure in dhx15^−/− zebrafish. A, cmyb expression of wildtype zebrafish at 3 dpf. B, B’, cmyb expression of Dhx15^−/−; Tg(hsp: DHX15) zebrafish at 3 dpf with (B’) or without (B) heat‐shock treatment. HSPC failure was rescued after DHX15 overexpression in dhx15^−/− zebrafish (P < .0001, χ² test). C‐C’’, cmyb expression of Dhx15^−/−; Tg(hsp: DHX15 R222G) zebrafish at 3 dpf with (C’, C’’) or without (C) HS treatment. HSPC failure was rescued after DHX15 R222G overexpression in dhx15^−/− zebrafish (P < .05, χ² test). DHX15 R222G is not as effective as DHX15 in restoring HSPC failure in dhx15^−/− zebrafish. (No HS vs HS, P < .05, χ² test; No HS vs HSL, P < .0001, χ² test; HS vs HSL, P < .005, χ² test). D, Western blot analysis of DHX15 protein expression in DHX15/DHX15 R222G transgenic zebrafish with or without heat activation. DHX15, Dhx15^−/−; Tg(hsp: DHX15); DHX15 R222G, Dhx15^−/− ; Tg(hsp: DHX15 R222G); dpf, days post fertilization; hpf, hours post fertilization; HS, heat‐shocked at 38℃ for 1 h and 45 min once a day since 25 hpf; HSL, heat‐shocked at 38℃ for 2 h once a day since 25 hpf; HSPC, hematopoietic stem/progenitor cell; No HS, without heat‐shock treatment. Black arrow indicates caudal hematopoietic tissue

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