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Fig. 5
Definitive hematopoiesis in zebrafish is normal when DHX15 or DHX15 R222G is overexpressed. WISH analysis of definitive hematopoietic markers: cmyb (A‐A’’), hbae1 (B‐B’’), mpx (C‐C’’), and rag1 (D‐D’’). Embryos from wildtype, Tg(hsp: DHX15), and Tg(hsp: DHX15 R222G) zebrafish were heat‐shocked at 38℃ for 2 h every day since 25 hours post fertilization (hpf). DHX15, Tg(hsp: DHX15); DHX15 R222G, Tg(hsp: DHX15 R222G). Black arrows indicate caudal hematopoietic tissue; red arrowheads indicate thymus. hpf, hours post fertilization; WISH, whole‐mount in situ hybridization
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