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Fig. 1
Generation of dhx15−/− zebrafish. A, DNA sequencing results of dhx15+/+ , dhx15+/− , and dhx15−/− embryos. Arrows indicate a 13 bp deletion (CTGAACCGCCATC) at dhx15 exon 2. B, B’, WISH analysis of dhx15 in dhx15+/+ and dhx15−/− embryos at 24 hpf. C, Western blot analysis of dhx15 in dhx15+/+ and dhx15−/− embryos at 3 dpf. D‐F’, Bright‐field microscopy images of dhx15+/+ and dhx15−/− zebrafish. dpf, days post fertilization; hpf, hours post fertilization; WISH, whole‐mount in situ hybridization
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