Figure 3

Whole organism imaging of H&E-stained adult zebrafish enables the visualization of histology-like cross-sections. (A) Representative sagittal cross-section of adult wild-type zebrafish resulting after 4h post-injection of 3.5% λ-CGN by i.p. (B–E) The histopathological lesions recorded on selected tissues. (B) The projection showing detail of liver damage with the arrow indicating a leukocytes infiltration in the hepatic fracture caused by the induced swelling. (C) Pancreas with inflammation and recruited leukocytes present in the connective tissue capsule. (D) Dorsal muscle fibers at the edge of the peritoneal cavity showing muscular edema, the arrows highlight the infiltrating cells. (E) Ventral muscles denoting a clear inflammatory process on top of the muscularis and besides muscle packs. (n = 10). For reference the swim bladder-SB is indicated. Scale bar 50 µm.