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Fig. 5
Figure 5. OPLS−DA score plot and loading scatter plot (A,B) of negative and positive (C,D) modes based on the zebrafish larvae normalized data exposed to 30 µM As2O3 in comparison to control larvae. (E) Differential expression of metabolites in As2O3-exposed larvae produced by hierarchical clustering of the most significantly upregulated (red) and downregulated (blue) metabolites obtained from in negative- and positive-ion modes compared to the control group based on the log2 fold change value. (F) Metabolic set enrichment analysis of lipid metabolites in 6 dpf zebrafish showed the biosynthesis of unsaturated fatty acids, arachidonic metabolism, and sphingolipid were dysregulated after embryonic exposure to As2O3. Color intensity (yellow–to–orange/red) represents increasing statistical significance, whereas circular diameter is related to pathway impact. The graph was obtained by plotting−log of p-values from pathway enrichment analysis on the y-axis and the pathway impact values derived from pathway topology analysis on the x-axis.
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