Fig. 4

Figure 4. Effects of embryonic As₂O₃ exposure on the color preference of larval (11 dpf, A) and adult zebrafish (B). (A) In the 11 dpf larval test, a cross maze with four different-colored cambers was used. Reduction in color preference for blue in As₂O₃-exposed larvae compared to the control group. No significant differences were observed in color preference for red and green in control or As₂O₃-exposed larvae. However, MK-801-exposed larvae showed no clear color preference (B) Five-minute video tracking of color preference in adult fish after associative learning. Adult fish were acclimated to the three-chamber maze for 6 days with red food association before the test on the 7th day. As₂O₃-exposed zebrafish showed no significant preference for either green or red color Data are presented as mean ± SEM, (∗ p ≤ 0.005). n = 30 larvae per group, n = 22 adults per group. ∗ Significance at p ≤ 0.05 between left/center/right and between two color arms for each test, n.s: not significant.