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Fig. 8
Loss of GR increases brain glucose uptake. (A) GRKO fish had significantly higher uptake of the fluorescent glucose analogue 2-NBDG in the brain than the WT. (B) The transcript levels of slc2a1a, slc2a1b and slc2a2 in the brain was lower in the GRKO fish compared to the WT, but the expression of slc2a3a and slc2a12 remained unchanged. (C) Loss of GR did not change the ratio of phosphorylated to total AMPK (M.W. 62 kDa) in the brain. (D) GRKO fish had significantly higher brain mass compared to the WT. E. Brain protein concentration was significantly higher in the GRKO fish compared to WT. Values are means ± SEM (Relative fluorescence units; RFU n = 5–6; Transcript abundance n = 6; AMPK expression n = 9; Brain wet weight n = 9; Brain protein concentration n = 7–9). ns—not significant; *(P < 0.05); **(P < 0.01).