ZFIN Image: Khrystoforova et al., 2022, Figure 1

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Figure Caption

Figure 1

CRISPR-Cas9 induced lrp5 zebrafish knockout line. (A) Schematic representation of the ZF lrp5 gene structure with Sanger sequence chromatogram; (B) demonstrating the del8bp (Ala53fs) mutation, that results in a frameshift and a premature stops codon; (C) Schematic representation of the ZF LRP5 wild-type and mutated protein demonstrates the lack of functional domains in LRP5 of lrp5^-/- (LY -low-density lipoprotein receptor; EGF – epidermal growth factor; LDLa- Low-density lipoprotein receptor domain class (A, D) Western-blot results demonstrated the absence of LRP5 protein in lrp5^-/-.(E) Each column in the graph represents the proportion of genotype (%) per developmental stage. Only 3.7% of lrp5^-/- in survive and reach adolescence using instead of expected 25%.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)