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Fig. 1
Zebrafish as a vertebrate model for scoliosis. Left: Different types of scoliosis and their potential causes from zebrafish studies. Scoliosis due to neuromuscular defects is also illustrated as CS like group. Asterisks indicate the abnormally developed vertebrae in CS-like zebrafish mutants. Right: Various bone staining and imaging methods used to evaluate skeleton development in zebrafish. At larvae stages, the notochord and vacuoles can be easily visualized via bright-field image (top left) or LysoTracker dye staining (top middle). Skeletal development in zebrafish can be visualized via alcian blue-Alizarin red double staining (top right). At juvenile or adult stages, skeleton development can be visualized via transgenic labeling, calcein staining, Alizarin red staining or micro-CT