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Fig. 1
Overexpression of zebrafish or human LGP2 facilitates the activation of fish and human promoters in fish cells but not in mammalian cells (A–D) DrLGP2 and HsLGP2 both activated fish IFN promoters (A, C) and human IFNβ promoter (B, D) in fish cells. EPC cells and CO cells seeded overnight in 24-wells plates were co-transfected with DrIFNφ1pro-luc or CaIFNpro-luc (A, C), or HsIFNβpro-luc (200 ng) (B, D), together with DrLGP2 (A, B) or HsLGP2 (C, D) at increasing doses (0, 10, 50, 100, 200 ng). pRL-TK (20 ng) was transfected as internal control. 48 h later, cells were collected for luciferase assays. (E, F) DrLGP2 and HsLGP2 did not activate fish IFN promoters (E) and human IFNβ promoter (F) in mammalian cells. HEK293T cells and COS7 cells seeded overnight in 24-wells plates were co-transfected with DrIFNφ1pro-luc or CaIFNpro-luc (E), or HsIFNβpro-luc (200 ng) (F), together with DrLGP2 or HsLGP2 at increasing doses (0, 10, 50, 100, 200 ng). Renilla vector (pRL-TK, 0.2 ng) was transfected as internal control. 48 h later, cells were collected for luciferase assays. Error bars show the SDs of triplicate transfections. Data were shown as mean ± SD (N=3). P values were calculated using ANOVA. **P < 0.01, *P < 0.05.