Fig. 6

Fig. 6

Fig. 6. Cluap1^stl839 mutants display body axis symmetry defects that are inherited at the Mendelian ratio and correspond to homozygosity of the mutant allele. (A) Representative images of control sibling and cluap1^{stl839/stl839} homozygous mutants at 4 dpf. Mutants display body curvature. (B) This curvature is present in 24.3% of total embryos, which suggests Mendelian inheritance of the mutant allele. (Statistics: Chi-square, observed vs. expected, p ​= ​0.75). (C) Image of the fragment analyzer gel output data. Lane ‘+’ is the positive heterozygous control for the 6-bp indel; lane ‘–‘ is the negative control. Lanes 1–14 are F2 embryos bred from an incross between F1 adult cluap1^stl839/+ carriers with the 6-bp indel. Lanes 1–7 represent embryos that look phenotypically normal. There is a combination of heterozygous (1–5) and homozygous wildtype (6, 7) alleles in the phenotypically normal group. Lanes 8–14 represent embryos with body curvature. All embryos with curved bodies genotype as homozygous mutants. Notice the slight variability between the sizing of bands in Lanes 1 and 12 compared to similar genotypes. Since each lane is run independently through an individual single capillary, there is a possibility for small sizing shifts between consecutive wells.