Figures for Neuffer et al., 2022


Figure Caption/Comments:

Fig. 4

Specification and early differentiation markers are unchanged in crasher (ap3d1 mutants). (a and b) Melanoblast/iridoblast bipotent precursor marker, foxd3, expression is unchanged in progeny of crasher heterozygotes. (c–h) mitfa expression is not altered in crasher heterozygote progeny. Melanoblast marker, mitfa, expression at 24 h postfertilization (hpf) in *AB control (c) and crasher heterozygote progeny (d) is not different. (e–h) Representative early differentiated melanophore marker, mitfa, expression at 30 hpf in *AB control (e) and crasher heterozygote progeny (f) from 2 replicates is not different. (g) mitfa + cell counts are not significantly different for early differentiating melanophores at 30 hpf between progeny of *AB parents and crasher heterozygote parents (Student's t-test, t(49) = 0.36, p = 0.72). (h) crasher heterozygote progeny have similar mitfa + signal, but less mitfa + signal on the ventral side. Ratio is mitfa + dorsal cell number divided by mitfa + ventral cell number (Student's t-test, t(45) = −0.94, p = 0.35). Plots are violin box plots. Scale bars are 500 μm. Images labeled crasher are representative fish from the clutch.

Figure Data:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Pigment Cell Melanoma Res.