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FIGURE 7
Wnt/β-catenin upregulation can rescue hypoxia-induced vascular defects. Upregulation of Wnt/β-catenin signalling using 40 µM SB216763 (SB) can rescue the vascular organization (reciprocal distance and vessel diameter) in hypoxia-treated embryos. Conversely, downregulation of Wnt/β-catenin signalling using 5 µM XAV939 leads to a worsened phenotype. Intersegmental vessels (arrows) were inspected in 3 dpf embryos, after 2-days long incubation in normoxia (control) or 5% hypoxia, exploiting the endothelial-specific transgene