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Fig. 1
Overview of three-dimensional behavioral tracking. (A) Individual fish were placed into a novel tank while video was recorded from above using D435 Intel RealSense™ cameras. (B) Videos included both a color (top) and a depth (bottom) stream where fish can be seen (yellow arrows). (C) Animals were tracked in the color videos using DeepLabCut to identify five points along the length of the fish. (D) Tracking was overlaid with the depth stream to generate a three-dimensional trace for each animal. (E) Four exploratory parameters were extracted from each trace: bottom distance (top left), center distance (top right), distance travelled (bottom left), and percent of the tank explored (bottom right).