ZFIN Image: Willekers et al., 2022, Fig. 5

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Fig. 5

Cfap53 is important for the localization of centrosomal components and centriolar satellites at the MTOC. (A-C, n=9) Maximal projections of confocal stacks from fixed Tg(ubi:GFP-cfap53) transgenic embryos immunolabeled for centrin at 2 hpf shows strong colocalization of GFP-Cfap53 with centrin during metaphase. (D-L) Maximal projections of confocal stacks from fixed wild-type or MPcfap53^−/− embryos at 45 mpf immunolabeled for centrosomal components. γ-tubulin (D; n=8; G, n=15; J, n=3), PCM1 (E, n=7; H, n=15; K, n=3) and centrin (F, n=5; I, n=8; L, n=14) in wild-type embryos (D-F), MPcfap53^−/− non-developing embryos (G-I) or MPcfap53^−/− developing embryos (J-L) at 45 mpf fixed. Scale bars: 20 μm. Schematics of embryonic development are adapted, with permission, from Kimmel et al. (1995).

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