Fig. 8

ctgfa expression increases at the injury site. (a–c) Representative images from stills of time-lapse movies of 5 or 6 dpf ctgfa:egfp;olig2:dsred larvae. Larvae were either uninjured (a; n = 4 nerves in 4 larvae), injured in muscle adjacent to spinal motor nerves (b; n = 4 nerves in 4 larvae), or injured along the spinal motor nerve (c; n = 11 nerves in 5 larvae) and time-lapse imaged for 6 hpi. Dashed circles indicate the injury site. White arrows indicate increased ctgfa expression. (d) Representative images from stills of a time-lapse movie of a 6 dpf nkx2.2a:gal4;UAS:NTR-mcherry;ctgfa:egfp larva (n = 6 nerves in 4 larvae). A single z-plane image demonstrates potential regions of co-localization of perineurial glia (magenta) and ctgfa expression (green) at 8 hpi. Solid white arrows follow bridging perineurial glia across the injury site. Open white arrows indicate ctgfa expression that might co-localize with perineurial glia. Open yellow arrows specify increased ctgfa expression that does not co-localize with perineurial glia. (e,f) Representative images from stills of time-lapse movies of 5 or 6 dpf ctgfa:egfp;olig2:dsred larvae. Larvae were either treated with 1% DMSO (E; n = 4 nerves in 3 larvae) or 10 μM SB431542 (F; n = 4 nerves in 3 larvae). White arrows follow the increase in ctgfa expression proximal to the injury site and yellow arrows follow the increase in ctgfa distal to the injury site. Scale bars, 25 μm; magnified insets, 10 μm.