ZFIN Image: Liu et al., 2022, Fig. 2

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Figure Caption

Fig. 2 Fig. 2. ISG15-Uba7 from basal vertebrates retain the ancestral Ub-Uba1 phenotype. (A) zUba1 activates Ub, zISG15. The activation was analyzed by immunoblotting with anti-Myc. (B) hUba1 could not activate Ub, zISG15, and hISG15. The activation was analyzed by immunoblotting with anti-Myc. (C) zUba1 activates ISG15s from lamprey, elephant shark, spotted gar, zebrafish. The samples were analyzed by immunoblotting with anti-HA. (D) zUba7 activates Myc-zISG15 and Myc-Ub. The activation was analyzed by immunoblotting with anti-Myc. (E) Activation of Ub by Uba7s from elephant shark, spotted gar, coelacanth expressed in bacteria. The samples were analyzed by immunoblotting with anti-Myc. (F) hUba7 could not activate Myc-hISG15 and Myc-Ub. The activation was analyzed by immunoblotting with anti-Myc. (G) Uba7 proteins of anole lizard, chicken, platypus, and opossum purified from HEK293T cells could not activate Ub. The activation was analyzed by immunoblotting with anti-Flag.

Acknowledgments

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