Fig. 3

Fig. 3

(A) Schematic diagram showing aqp8ab sgRNA targeting site (indicated by arrowhead) in the third exon of aqp8ab gene. Starting codon (ATG) site is indicated by arrow. The sgRNA targeting sequence is highlighted in brown and the PAM in purple. (B) Mutation pattern of mRNAs-injected embryos. Numbers in the brackets show the number of nucleotides that were deleted (–) or inserted (+). Inserted nucleotide is in red, changed nucleotide is in blue. WT, wild-type. (C) Three heritable mutants were identified by screening. F0 offspring were out-crossed with wild-type fish to produce F1, and the DNA extracted from tail fins of F1 adults were used for the identification of heritable mutants by sequencing. (D) Schematic diagram showing the predicted proteins encoded by the three mutated alleles. The mutants are reading frameshift mutations that result in truncated proteins.