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Fig. 3

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ZDB-IMAGE-220725-28
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Figures for Osorio-Méndez et al., 2022
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Fig. 3

scn8ab Is dispensable during early development. (A) Number of zebrafish with scn8ab+/+, scn8ab+/Δ, and scn8abΔ/Δ genotypes scored at 5 dpf (Left) and at 3 mpf (Right). The observed genotypic ratio matched the expected ratio in 5 dpf larvae from scn8abΔ/+ in-crosses, but no scn8abΔ homozygotes recovered at adult stages (>3 mpf). (B) Representative whole-mount images of control (heterozygous sibling) and temca at 3 mpf. (C and D) Quantification of body weight (C) and body length (D) at 3 mpf (n = 41 and 53 for scn8abtemca/+ and scn8abtemca/Δ, respectively). (E) Control (heterozygotes) and temca 6 dpf larvae swimming tracks at 34 °C. (F) Quantification of swimming behavior of 6 dpf control and temca larvae (n = 16 and 20 for control and temca, respectively). Two-way ANOVA, temperature [F(1,33) = 0.01992, P = 0.889]; genotype [F(1,33) = 0.005127, P = 0.943]; genotype × temperature interaction [F(1,33) = 4.119, P = 0.051]. (G) Representative images of control (heterozygotes) and temca regenerated fin folds 3 dpa at 33 °C. (H) Quantification of 3 dpa larvae fin fold–regenerate lengths at 33 °C (n = 13 and 17 for control and temca, respectively). Scale bars, 1 cm in B, 200 µm in G. (C, D, and H) Data are presented as mean ± SD by Student’s unpaired two-tailed t test.

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