The indicated cells were left unstimulated or stimulated with 10 ng/ml VEGF (a, b) or 30 ng/ml FGF2 (c) with UniPR1331 (30 μM in a–c or increasing concentrations in b). Then, cells were analyzed by WB with anti-P-VEGFR2 (a, b) or anti-P-FGFR1 (c) antibody. Uniform loading was confirmed with anti-VEGFR-2 or anti-tubulin antibody. The results shown are representative of other two that gave similar results. The single lanes have been cropped and reorganized for an easy comparison. b Densitometric quantification of P-VEGFR2 immunoreactive bands normalized to the expression levels of VEGFR2. (mean ± S.E.M. of three independent experiments, *P < 0.005).
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