ZFIN Image: Rusnati et al., 2021, Fig. 2

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Figure Caption

Fig. 2 Effect of UniPR1331 on VEGF/VEGFR2 binding at the cell surface.

a Chlorate-treated ECD-VEGFR2-EYFP GM7373 cells were incubated with VEGF and UniPR1331 and analyzed in immunofluorescence with anti-VEGF antibody. Fluorescence was quantified using ImageJ software as corrected total cell fluorescence: integrated density − (area of selected cell × mean fluorescence of background). Data are the mean ± S.E.M. of measurements on 25–35 cells for each sample from two independent experiments. (*P < 0.0001 in respect to cell treated with VEGF alone). b Microphotographs of ECD-VEGFR2-YFP GM7373 incubated with VEGF and UniPR1331. Scale bar: 10 μM. c ECD-VEGFR2-YFP A745 CHO cells were added to HSPG-bearing CHO-K1 monolayers with VEGF and UniPR1331. Adherent cells were photographed and counted after 2 h of incubation. Data are the mean ± S.E.M. of cell count in six microscopic fields. d Microphotographs of ECD-VEGFR2-YFP A745 CHO-K1 incubated on HSPG-bearing CHO-K1 monolayers with VEGF and UniPR1331.

Acknowledgments

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