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Fig. 3
Over expression of ACE2 in HEK293T cells. Cultured HEK293T cells were transfected with cDNA encoding ACE2 for 48 h before being immunolabeled with an anti-ACE2 antibody (in green). The cell nucleus was labelled with DAPI (in blue), while the cytosol and plasma membrane were stained via the expression of ATPase (in red). The cells were visualized, and the images were acquired with a Leica SP8 laser scanning confocal microscope. In each panel, the region indicated by the asterisk is shown in a higher magnification in the inset panel. When the data were quantified, the results showed that >50% of the HEK293T cells had successfully been transfected with ACE2 cDNA. The data represent the mean ± SD; n = 4. The statistical differences were detected utilizing the two-sample t-test, *** p < 0.001.