FIGURE 1

FIGURE 1

Phenotypes of different mutants at various stages. (A) Generation of eomesa^tsu007 mutant allele. Left: genomic structures and putative coding products of eomesa WT allele and tsu007 allele. The exons were colored and positions of nucleotides and amino acids were indicated. The mutant allele carries a 353-bp deletion. Right: morphology of eomesa^+/tsu007 (heterozygote) and eomesa^{tsu007/tsu007} (zygotic mutant) adults. Note that the posterior dorsal fin (indicated by an hollow arrowhead) is absent in mutant adult. (B) Scheme of generation of Mhwa;Meomesa double mutants. Zygotic genotypes were indicated. Zhwa^−/−;Zeomesa^−/− homozygous female adults could not ovulate naturally, and eggs squeezed from these fish were used for in vitro fertilization (IVF) using sperms squeezed from wildtype males (Zhwa^+/+;Zeomesa^+/+). (C) Wildtype (WT) embryos at indicated stages. (D,D’) Meomesa mutants. Mutant embryos exhibited variable phenotypes at 8 hpf and 24 hpf, and the ratios of 24-hpf mutants categorized into different classes (I-III), which were derived from several homozygous females, were shown in (D’). (E) Mhwa mutants at indicated stages. (F,F’) Meomesa;Mhwa double mutants. The ratios of embryos with different phenotypes at 24 hpf were shown in (F’). Embryos were laterally positioned when the dorsal or tail was recognizable. The scale bar in (C) was also applied to (D–F).