Fig. 5

Figure 5. GSCs contribute to the germline cell lineage of host embryosto generate zebrafish germline chimeras. (A) The dnd MO abolished the expression of dnd-GFP reporter, whereas the control MO did not. (B) Images showing GFP signals in embryos injected with control and dnd MOs. The GFP-positive PGCs (red arrows) were distributed in the gonadal region of the Tg(piwil1:eGFP) embryos or embryos injected with control MO. No GFP-positive PGCs were observed in the gonadal region of embryos injected with dnd MO. The frequency of embryos with the indicated phenotypes was shown in the bracket of each group. Lateral view. Scale bars, 500 μm. (C) Gel images of RT-PCR products showing the expression of the eel-specific dazl gene in gonads of transplanted zebrafish. β-actin was used as a reference gene. Red arrows indicate PCR bands amplified by second-round RT-PCR. (D) Alignment of the amplified dazl sequences from gonads of M. albus and gonads of three fGSCs-transplanted zebrafish. (E) IF staining of Vasa in ovaries of control-, dnd MO-, and dnd MO-transplanted with fGSCs zebrafish. White arrows indicate the germ cells. Scale bar = 50 μm.