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Fig. 2
Figure 2. Isolation and characterization of GSCs derived from ovary and testis. (A) Cell morphology of GSCs isolated from ovary and testis at the indicated time points. Red arrows show the grape-like GSCs, and the white arrows show the blood cells. (B) AP staining images of fGSCs and SSCs. (C) qRT-PCR results of pluripotency- and GSC-specific genes in the isolated fGSCs, interGSCs, and SSCs. The spleen was used as the non-gonadal control. (D) Immunofluorescence staining results of Nanos2 and Vasa in fGSCs and SSCs.
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