Fig. 2

Figure 2. Expression of nos1 in medaka embryos and larvae. At stage 28, whole-mount in situ hybridization experiments displayed positive cells in the rostral portion of the nervous system and in the tailbud (A,B). At this stage, sections showed signal around the diencephalon-hypothalamus boundary (1–2), in the extraocular muscle (2–3), and caudally in the thyroid primordium (4). At stage 30 (C,D), expression was retained in the extraocular muscle and thyroid primordium, (5 to 9) and nos1-positive cells were detected in ventricular layer cells of the rhombencephalon (8–9), between the alar and basal plate (9), and in the rhombencephalic area (10). At stage 32 (E,F), nos1 was expressed in the subpallial and pallial region (11–12), in the extraocular muscle (13–15), and the thyroid primordium (15–16). Caudally, nos1 signal was present in the rhombencephalon (17–18), notochord, and sympathetic ganglia (18–19). At stage 34 (G,H), hybridization signal was present in the subpallium, preoptic area, hypothalamic domains, thalamus, optic tectum, eye and extraocular muscle (21 to 25), basal and alar plate of the rhombencephalon (26 to 28), notochord, and liver primordium (28). Abbreviations: ap, alar plate; bp, basal plate; phb, prethalamus-hypothalamus boundary; em, extraocular muscles: tp, thyroid primordium; h, hypothalamus; lv, liver; n, notochord; ot, optic tectum; pn, pallium nuclei; r, rhombencephalon; rbp, rhombencephalic basal plate; re, retina; rp, rhombencephalic plate; rvl, rhombencephalic ventricular layer; s, subpallium; sg, sympathetic ganglia; t, thalamus; tp, thyroid primordium. DV dorsal view; VV, ventral view; LV lateral view. Scale bar: whole-mount 100 µm; sections 250 µm.