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Fig. 4
Validation of segmentation sensitivity. (A-B) Data were acquired before and after exsanguination. (C) Vascular volume was not statistically significantly different when comparing controls to exsanguinated samples after GF (p 0.2596; n = 16; 4 dpf embryos; two experimental repeats; paired t-test). (D) Vascular volume showed a statistically significant decrease when comparing controls to exsanguinated samples after SE (p < 0.0001; paired t-test). (E) CNR did not show a statistically significant change in the exsanguination procedure (p = 0.0876; paired t-test).
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