Fig. 7

Fig. 7

A Examples of iGluSnFR signals from individual OFF synapses elicited using 60% contrast stimulus (5 Hz, 30 s) in the morning (top), afternoon (middle) and afternoon + SCH 23390 (bottom). In each case the top trace shows the iGluSnFR signal and the lower trace the estimated Q_e.B Changes in Q_e in ON synapses in the morning (light grey bars, n = 10 synapses) and afternoon (green bars, n = 14 synapses). In the afternoon the distribution was shifted toward multiquantal events (p < 0.05, KS-test). C Changes in the distribution of Q_e in ON synapses before and after intravitreal injection of the D1 antagonist SCH23390 (dark grey bars, n = 8 synapses). The distribution was shifted toward lower Q_e (p < 0.001) but was not significantly different to that measured in the morning. D Changes in Q_e, in OFF synapses in the morning (light grey bars, n = 19 synapses) and afternoon (red bars, n = 24 synapses). In the afternoon the distribution was shifted toward multiquantal events (p < 0.02). E Changes in the distribution of Q_e in OFF synapses before and after intravitreal injection of SCH 23390 in the afternoon (dark grey bars, n = 12 synapses). The distribution was shifted toward uniquantal events (p < 0.001). Source data are provided as a Source Data file.